PONCEAU S FOR ELECTROPHORESIS
Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protei
- Usage unit
- Box
Description
Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.
Application
Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*.
Notes
Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.) Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v).
Procedure
Place the blot transfer membrane in a plastic box and rinse it with water three times, 5 minutes each. Stain the membrane with Ponceau S stain for 30 seconds to 1 minute. Destain the membrane with several changes of water for 30 seconds to 1 minute each, then air dry
Other names
PONCEAU S FOR ELECTROPHORESIS
Packing & supply
Supply locations
[PONCEAU S FOR ELECTROPHORESIS] manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman. Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada. [PONCEAU S FOR ELECTROPHORESIS] is available in small packing as well as in bulk. Buy premium quality [PONCEAU S FOR ELECTROPHORESIS] and other lab chemicals from one of the most trusted brands.
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