TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE
Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.
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Description
Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.
Application
Tricine gels are suitable in isolating hydrophobic proteins from 2D gel for mass spectrophotometric analysis. It is also helpful to isolate membrane protein complexes from biological membranes. When urea is added in stacking gel it can easily separate two different proteins of the same molecular weight
Notes
Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived. It is a white crystalline powder that is moderately soluble in water. It is a zwitterionic amino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15.
Procedure
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous
Other names
TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE
Packing & supply
Supply locations
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